diff --git a/src/main/java/org/micromanager/lightsheetmanager/gui/tabs/acquisition/CameraPanel.java b/src/main/java/org/micromanager/lightsheetmanager/gui/tabs/acquisition/CameraPanel.java
index 435b777a..1df86e96 100644
--- a/src/main/java/org/micromanager/lightsheetmanager/gui/tabs/acquisition/CameraPanel.java
+++ b/src/main/java/org/micromanager/lightsheetmanager/gui/tabs/acquisition/CameraPanel.java
@@ -168,6 +168,10 @@ private void computeCameraOrder() {
         // update camera order
         model_.acquisitions().settingsBuilder()
                 .imagingCameraOrder(cameraData.toArray(CameraData[]::new));
+        // live mode and the slice timing read the primary camera from the built settings, and
+        // simultaneous cameras can have different reset and readout times, so rebuild the
+        // settings and recompute the timing now
+        model_.acquisitions().updateDurationLabels();
     }
 
     @Override