TotalSync

TotalSync is a low-cost, open-source teensy-based synchronization system for multiple recording techniques.

uv tool install totalsync

Read the documentation →

Getting started

• General Introduction to TotalSync
• Installation
• Quick start
• Pin usage
• Extending TotalSync for new devices
• Videos and images

Command reference

• totalsync — record a session and serve the live browser interface
• totalsync-decode — decode recorded .b64 files into numpy, MATLAB or pynapple formats, with named channels
• totalsync-pinsheet — generate the pinSheet.json channel map from the Teensy firmware source
• totalsync-2p — align ScanImage two-photon recordings with TotalSync telemetry

Recording techniques

• Voltage sensitive imaging

Chapters for two-photon microscopy, electrophysiology / Neuropixel and virtual reality are planned; their placeholder files under docs/ are not part of the site yet.

Repository layout

This is a uv workspace of four distributions. pip install totalsync gets all of them; each is also installable on its own, so a machine that only analyses recordings need not pull in the serial and GUI dependencies.

Path	Distribution	Provides
packages/totalsync_webinterface	totalsync-webinterface	totalsync — acquisition, recording and the live browser interface
packages/totalsync_utils	totalsync-utils	totalsync-decode, totalsync-pinsheet
packages/totalsync_2p	totalsync-2p	totalsync-2p
.	totalsync	umbrella that depends on the three above

Teensy41_Totalsync/ holds the firmware, and docs/ the documentation above.

Contribute

Please contact Morgane Audrain (morgane.audrain@donders.ru.nl) with questions or concerns.